瘦素对牛体外胚胎早期发育阻滞的克服

doi:10.11843/j.issn.0366-6964.2014.05.012

Abstract

Abstract:

The experiment was conducted to study Leptin on in vitro early embryonic development diapause.The different concentration of Leptin 0,1，10,and 100 ng·L^-1 were added to IVF early embryo medium,using 1% FCS as control group.The bovine in vitro embryos development with different concentration of Leptin were observed.RT-PCR was used to detect the expression of Leptin mRNA of normal and arrested embryo and the effect of Leptin on L-R and STAT3 mRNA expression.The results showed that: （1） Embryo cleavage rate and blastocyst rate of addition 10，100 ng·L^-1 Leptin is significantly higher than control group (P<0.05); （2）The expression of Leptin mRNA in normal embryo was significantly higher than that of arrested embryo （P<0.05）; （3） The expression of L-R,STAT3 mRNA of in vitro early embryo with exogenous Leptin was significantly higher than that of the control one（P<0.05）. In conclusion， Leptin is very important to bovine in vitro early embryonic development, less expression of Leptin and L-R is mark to embryonic development diapause, and these process play a role in JAK/STAT3 pathway.

CLC Number:

S823
S814.8

YAN Peng， NI He-min， LIU Yun-hai， GUO Yong， LIANG Lin， ZHENG Xue-ying. Effects of Leptin on in vitro Early Embryonic Development Diapause[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(5): 763-768.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2014.05.012

https://www.xmsyxb.com/EN/Y2014/V45/I5/763

References

［1］BOWMAN P，MCLAREN A.Cleavage rate of mouse embryos in vivo and in vitro［J］.Embryol Exp Morph, 1970,24(1):203-207.
［2］李逸平,左嘉客.哺乳类早期胚胎发育阻滞的形成与突破［J］.细胞生物学杂志，1992,14(4)：153-158.
［3］IOANNIS E M，SPYROS D M.Leptin in human reproduction［J］.Hum Reprod,1999,5(1)：52-63.
［4］郝翠芳，郎翠红.IL-1β和瘦素在胚胎着床中对ICAM-1和MMP9的影响［J］.中国妇幼保健,2011，26：2995-2999.
［5］MALIK N M，CARER N D，MURRAR J F，et al.Leptin requirement for conception，implantation and gestation in the mouse［J］.Endocrinology，2001，142(12)：5198-5202.
［6］LI X X,LEE D S,KIM KEUM J， et al.Leptin and nonessential amino acids enhance porcine preimplantation embryo development in vitro by intracytoplasmic sperm injection［J］.Theriogenology,2013，79(2)：291-298.
［7］KAWAMURA K，SATO N，FUKUDA J, et al.Leptin promotes the development of mouse preimplantation embryos in vitro ［J］.Endocrinology，2002，143（5）:1922-1931.
［8］BASAK S，DUTTAROY A K.Leptin induces tube formation in first-trimester extravillous trophoblast cells［J］.Eur J Obstet Gyecol Reprod Biol，2012,164(1)：24-29.
［9］龚姗.瘦素在人类生殖领域的研究现状［J］.计划生育杂志,2011,30(4)：334-337.
［10］CHAKRABARTI J ，CHATTERJEE R，GOSWAMI S,et al.Overt leptin response to controlled ovarian hyperstimulation negatively correlates with pregnancy outcome in vitro fertilization--embryo transfer cycle［J］.J Human Reprod Sci,2012,5(2):194-199.
［11］李拥军，敖红,孙桂金.影响羊早期胚胎发育阻滞相关基因的筛选［J］.扬州大学学报,2007，28（4）：25-28.
［12］梅承,施振旦,吴嫦丽.Leptin 和ITS 对牛卵母细胞体外培养和胚胎生产的影响［J］.中国兽医学报，2008，28（4）：475-479.
［13］BOELHAUVE M，SINOWATZ F，WOLF E.Maturation of bovine oocytes in the presence of Leptin improves development and reduces apoptosis of in vitro-produced blastocysts［J］.Biol Reprod,2005，73:737-744.
［14］宗兴平,严宗哲,陈援农，等.瘦素和瘦素长型受体在胚胎停止发育中的表达［J］.现代妇产科进展,2008,17:654-657.
［15］GONZALEZ R R，CABALLERO C P，JASPER M,et al.Leptin and leptin receptor are expressed in the human endometrium and endoetrial leptin secretion is regulated by the human blastocyst［J］.J Clin Endocrinol Metab，2000，85:4883-4888.

[1]	CAO Jianhua, YANG Baigao, ZHANG Peipei, FENG Xiaoyi, ZHANG Hang, YU Zhou, NIU Yifan, HAO Haisheng, DU Weihua, ZHU Huabin, YANG Ling, ZHAO Xueming. Mechanisms of Negative Energy Balance Affects Follicular Development in Dairy Cattle [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 22-30.
[2]	MIAO Shu, AN Jishan, WANG Zuo, XIAO Dingfu, LAN Xinyi, LIU Lei, SHEN Weijun, WAN Fachun. Leucine Promotes the Proliferation of Bovine Myoblasts through PI3K-AKT Signaling Pathway [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 142-152.
[3]	LIU Yili, TANG Jiao, MIN Qi, YANG Lu, WANG Zening, HU Lian, ZHAO Di, JIANG Mingfeng. Mining Key Candidate Genes of Development and Metabolism in Yak Abomasum Based on Transcriptome Data [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 153-168.
[4]	YAO Ying, ZHOU Yingcong, DU Peiyan, LI Yijuan, QIAN Wenjie, LI Liuyang, YU Zhipeng, CUI Yan, YU Sijiu, FAN Jiangfeng. Proteomic Analysis of Yak Serum During Pregnancy Based on TMT Technology [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 192-206.
[5]	ZHANG Ying, YUAN Ligang, CHEN Guojuan, ZHANG Fang, YANG Dapeng. Analysis of Differential Expression of ET-1/eNOS in the Development of Cryptorchidism in Yak [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 207-217.
[6]	ZHANG Wentao, LIU Chenyang, ZHU Binglin, LIU Li, TIAN Yuan, YAO Yuhang, CHENG Gong. Study on the Effects and Mechanisms of Snail1 on Proliferation and Differentiation of Bovine Adipocytes [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5008-5019.
[7]	NIU Yifan, YANG Baigao, ZHANG Peipei, ZHANG Hang, FENG Xiaoyi, CAO Jianhua, YU Zhou, HAO Haisheng, DU Weihua, ZOU Huiying, ZHU Huabin, MA Youji, ZHAO Xueming. Advances in Bovine Embryo Genome Selection [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4449-4457.
[8]	WANG Wenxiang, DU Lili, HU Junwei, ZHANG Yanxiang, MA Minghao, DUAN Rui, QIAN Cong, WANG Xinyue, LI Sanlu, ZHANG Changqing, ZHANG Lupei, GAO Xue, XU Lingyang, LI Junya, GAO Huijiang. Mining Candidate Genes Related to Meat Quality Traits of Longissimus Lumborum in Pingliang Red Steer Based on Transcriptome [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4589-4604.
[9]	ZHANG Qian, CUI Yan, YU Sijiu, HE Junfeng, PAN Yangyang, WANG Meng. Transcriptome Analysis of the Cerebral Cortex in Newborn and Adult Yaks (Bos grunniens) [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4605-4614.
[10]	DONG Zhihao, SHI Yuxin, GUO Guanhua, YUAN Kaimin, XIU Haoyu, WANG Chao, BAI Junyan, WANG Dong. Comparative Analysis of Salivary Compounds in Different Estrous Stages of Cows [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4636-4652.
[11]	SUN Dongxiao, ZHANG Shengli, ZHANG Qin, LI Jiao, ZHANG Guixiang, LIU Chousheng, ZHENG Weijie. Application Progress on Genomic Selection Technology for Dairy Cattle in China [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4028-4039.
[12]	FENG Xiaoyi, HAO Haisheng, DU Weihua, ZHU Huabin, CUI Kai, ZHAO Xueming. Progress in Mechanism of Reduced Fertility in Dairy Cows due to Negative Energy Balance [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4050-4060.
[13]	MA Haoran, ZHANG Lupei, JIN Shengyun, BAO Jinshan, LI Hongyan, GAO Huijiang, XU Lingyang, WANG Zezhao, LI Junya. Assessment of the Genomic Relationships for Chinese Indigenous Beef Cattle Using High-density SNP Chip [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4174-4185.
[14]	GUO Yixin, WANG Zhisheng, HU Rui, WANG Junmei, WANG Sen, SHI Liyuan, ZHANG Xiaohong, ZOU Huawei, ZUO Jiaxue, PENG Quanhui, XUE Bai, WANG Lizhi. Effect of Leucine on Browning of Subcutaneous Adipocytes in Yellow Cattle [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3286-3298.
[15]	CHU Chu, ZHANG Jingjing, DING Lei, FAN Yikai, BAO Xiangnan, XIANG Shixin, LIU Rui, LUO Xuelu, REN Xiaoli, LI Chunfang, LIU Wenju, WANG Liang, LIU Li, LI Yongqing, JIANG Han, LI Weiqi, SUN Wei, LI Xihe, WEN Wan, ZHOU Jiamin, ZHANG Shujun. Establishment and Application of Prediction Model of Three Amino Acids in Milk Based on Mid-infrared Spectroscopy [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3299-3312.

Effects of Leptin on in vitro Early Embryonic Development Diapause

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments